This wide range of critical features facilitates microfluidic devices
design and makes the custom microfluidics platform highly flexible and ready for use in a large variety of applications. Fig. 2a
shows some of the fabricated components.
Pumps and valves are based on the stack of several layers of
conductive polymer actuators, actuation voltage is low (1-2 V)
thus enabling mobile, battery powered applications. The pumps
(Figure 2b) generate a high pressure ( 10-50 MPa); a sizeable flow
can hence be sustained even in structures with large fluidic impedance.
The actual diagnostic test is initiated by injecting an individual’s blood sample into the chip, and subsequently placing
Figure 2a.Photographs of some silicon fluidic structures. Indicated
in the figure are a meandered mixer, a cavity for performing PCR,
a coarse filter for DNA purification (pillars are 200 µm deep with a
spacing of 3-5 m), a filter for DNA separation (pillars are 50 µm deep
with a spacing of about 1 m). 2b. Photograph of fabricated conductive
polymer valve (top) and pump (bottom).
For more information, go to www.panabat.com/913-1 or call 1-877-PANABAT (1-877-726-2228).
Panasonic batteries power devices essential to the day-to-day
operations of medical facilities worldwide.
Our CR lithium cylindrical cells feature extraordinary reliability,
long service life, and high current discharge capability. Preferred for
applications like insulin pumps, AED, portable surgical tools or devices
requiring RF communications.
Our CR lithium coin cells are ideal for applications like iontophoresis devices, glucose meters, emergency call buttons and memory
Our BR-A lithium coin cells are ideal for memory backup and RF
communications— they feature high operating temperature, high
reliability, and long service life.
Our Lead Acid VRLA provide maintenance-free, long service life, as
well as quick chargeability for applications such as backup power,
wheelchairs and emergency lighting.
Keeping you in touch with your devices
the chip into the benchtop instrument. Once inside the chip,
the blood is mixed with pre-stored reagents and passed into a
microreactor. Here, the cells are lysed by applying a temperature
of 95°C for two minutes, their genetic content is released and the
SNP containing regions in the DNA are cut out and amplified
through a targeted PCR reaction. We have achieved high-speed
PCR, where 30 temperature cycles are completed in only nine
minutes. This result is due to the careful design of the heating
and cooling system (based on a commercial, microfabricated
thermoelectric cooler, Micropelt) and to the small microreactor
thermal mass obtained thanks to the trenches which separate it
from the rest of the chip. The amplified DNA is then purified by
retaining cell debris in an integrated sieve (Figure 2b). The part of
the chip so far described is a simple and effective ‘sample preparation module’ of very generic use.
Purified DNA is sent to the detection section of the chip where
it is mixed with specific reagents and a second PCR reaction is
performed. Primers are designed in such a way that amplification
occurs only if the SNP is present. The pyrophosphoric acid produced during this second PCR is later detected electrochemically.
The two-PCR process needs a combined optimization to avoid the
primers and phyrophosforic acid related to the first PCR influence, the second PCR reaction, and the detector reading.
The electrochemical detector is a small cavity (0, 5, 1 µL vol-
ume), containing three electrodes implemented in the polymer part
continues on page 25